Tips & Tricks For Successful Vitrification


An effective cryopreservation strategy is now the mainstay of fertility clinics all over. Importantly, as ‘freeze all’ cycles are slowly making gains, the necessity of a successful cryopreservation strategy is the order of the moment.

Vitrification has been the choice lately for cryopreservation and can differ slightly based on the temperature, cryo devices and the manufacturer of the media. Nevertheless, the basic principle and procedure is similar to all these factors. Vitrification procedure is accomplished using various Vitrification mediums available. The embryos are subjected to the ES (Equilibration media) before being incorporated into VS (Vitrification media) before being transferred into a cryo machine and then sucked into nitrogen liquid. An embryologist’s perspective, it has always been found that Vitrification is a lot more difficult to master than ICSI or other methods. Unfortunately, there isn’t an exhaustive learning module with respect similar. Fertility clinics don’t have the standard program of training to prepare for Vitrification. This can result into incompetency of the operator or the patient, as well as low survival rates and fluctuations in results!

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Vitrification of oocytes

The last step in embryo loading is at the heart of the whole process, and the entire Vitrification program is built upon the foundation of that vital last one minute. Naturally, the primary focus is on the final phase, which includes tasks such as washing embryos using the Vitrification System, loading them up with minimal media on the cryo device and emptying the media of excess and dumping into nitrogen liquid. This process is not too complicated, but it is definitely meticulous considering the microscopic aspects that go along with the race that takes place over time.

The greatest challenge with Vitrification is the combination of speed with time! But, this technique can be successfully implemented following an extensive stepwise way to learn it by self-devising an effective training environment.

When you first get acquainted with the technique simply learning the protocol isn’t enough. There must be an knowledge of the fundamental science behind it. People have been panicking when they see embryos shrinking ES!

Secondly, for starters it is suggested for embryologists to be knowledgeable about the different components of the Vitrification process. The purpose here is to do things perfectly without time constraints. The final step could be divided into small steps to aid in adaptation. The embryologist is able to adjust the fine knob on the microscope to pick up the embryos after they float owing to change in viscosity within VS. This includes the focusing of the cryotop under microscope, the position of the hands, learning how to wash, and finally , learning how to load the embryos!

Thirdly, once you have achieved the perfect technique, time will come into the picture. Then the use of stop watch and practicing loading the required amount of time becomes the focus. Initially, the loading time might be over two minutes. After a few weeks of practice you’ll be able to reduce the time needed and eventually place yourself in the stipulated interval of 60 to 90 seconds! The practice can be performed using degenerated oocytes.

Fourthly, after you’ve been able to manage the situation, the next natural factor will be survival! This is the time when you can use the embryos you have discarded and then freeze them exactly in the same way as you would an actual procedure. In this case, the ES can be integrated into the practice and you’ll be able to observe complex details like the reactions of embryos to the media. At this point, the you can also use thawing methods integrated as it contributes in the general survival. The outcomes can be recorded and give an insight into the overall progress of the operator.

Finally, once you are finished with the survival of embryos it is always recommended to freeze embryos to be used for donors since an ED cycle contains the highest embryos of high-quality. Blastocysts should be considered more in the initial phase of freezing as they have a better chance of survival than say day two or day three embryos. This can be furthered by increasing the number of cryo devices progressively while assessing the performance of previously frozen cryo devices at the same time.

This complete method of learning that process can take between six and six months overall depending upon the cycle happening in a fertility clinic , which certainly affects the availability of gametes and resources(such as expired media for practicing). It is a great way to gain knowledge of the process and the program is able to be rigorously standardized in the actual facility of fertility. The process of vitrification can allow embryos to hibernate into an ice age and maybe we could make their journey smooth!

Learn process of vitrification of embryos, Advanced course in cryopreservation, IVF training for Gynaecologist and Embryologist, Hands-on IVF IUI ICSI training courses and how to set up IVF and IUI Laboratory in India for Gynaecologist and Embryologist at

Embryology Academy for Research & Training is an IVF training institute in the field of assisted reproduction techniques that provides short course and hands on training to embryologists and young aspirants. We at EART with our well-balanced mix of various teaching modules, lectures, actual hands-on-training and demonstrations will provide you with a high-quality education that will leave you satisfied. At EART our aim is to provide the most innovative and competitive training available in the discipline of ART. Our customized training program since its inception in the year 2003, has had the honor of training over hundreds of international and national participants. EART is recognized as a research institution for IVF Training program for IVF or ICSI/ART Micromanipulation, Reproductive Medicine.


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